Potensi Penghambatan Enzim Tirosinase dengan Ekstrak Etanol Terstandar dan Fraksi Etil Asetat Kulit Bengkoang (Pachyrhizus erosus L.

Penulis

  • Zainur Rahman Hakim Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia ,
  • Ratna Djamil Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia ,
  • Novi Yantih Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia ,
  • Ocitania Sanasih Faculty of Pharmacy, Universitas Pancasila, South Jakarta, Jakarta 12640, Indonesia ,
  • Benni Iskandar School of Pharmacy, Faculty of Pharmacy, Taipei Medical University, No.250, Wuxing St., Xinyi Dist., Taipei City 110, Taiwan ,

DOI:

https://doi.org/10.35814/jifi.v21i1.1397

Kata Kunci:

Inhibisi enzim tyrosinase, kulit bengkoang (Pachyrhizus erosus L.), standarisasi ekstrak

Abstrak

Penelitian ini mengkaji potensi daya hambat tirosinase ekstrak etanol terstandar dan fraksi etil asetat kulit bengkoang. Sebanyak 450 g serbuk kulit bengkoang (Pachyrhizus erosus L.) dimaserasi dengan etanol 96% selama 2 x 24 jam, disaring, dan dipekatkan hingga kental. Standardisasi ekstrak menggunakan parameter spesifik dan non-spesifik. Tes penghambatan tirosinase dilakukan secara kolorimetri dalam lempeng mikro. Hasil determinasi tanaman mengidentifikasi kulit bengkoang sebagai Pachyrhizus erosus (L.) Urb. Uji organoleptik menunjukkan ekstrak kental berwarna kuning kecoklatan, bau segar, dan rasa pahit. Ekstrak larut etanol 47,60% dan larut air 22,42%. Ekstrak etanol kulit bengkoang meliputi alkaloid, flavonoid, saponin, tanin, dan steroid. Parameter non spesifik menunjukkan susut pengeringan (9,71%), kadar air (7,28%), abu total (9,91%), abu tidak larut asam (3,68%), tidak ada kandungan residu pelarut, kandungan timbal (-0,2324±0,1729), kandungan kadmium (-0,0841±0,3418), TPC 1 x 101 CFU/g, dan TYMC 2,26 x 102 CFU/g. Ekstrak etanol (103,9263) dan fraksi etil asetat (81,8606) nilai IC50 kulit bengkoang menurunkan aktivitas enzim tirosinase dibandingkan kontrol (25,1235 ppm). Hasil tersebut menunjukkan bahwa ekstrak etanol memenuhi standar baku mutu spesifik dan nonspesifik. Fraksi Etil asetat kulit bengkoang menunjukkan penghambatan yang cukup terhadap enzim tirosinase.

Referensi

Unduhan

Diterbitkan

2023-04-30

Terbitan

Vol 21 No 1 (2023): JIFI

Bagian

Articles

Lisensi

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